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CPT 0003U: Overa™ (OVA1 Next Generation) Ovarian Cancer Risk Assay

CPT code 0003U identifies Overa™ (OVA1 Next Generation/MIA2G), a proprietary multivariate index assay for assessing ovarian cancer risk through measurement of five serum biomarkers combined with algorithmic patient-data analysis. As a PLA code tied to a specific manufacturer and laboratory, it standardizes reporting for this unique diagnostic service and supports claims processing and utilization tracking nationally. Key payers in this analysis include Aetna, Blue Cross Blue Shield, Cigna Health, UnitedHealthcare, and Medicare.

Readers will find a concise overview of the code’s clinical role in ovarian cancer risk assessment, expected service setting, and the payer landscape. The publication summarizes benchmark coverage patterns, coding and billing considerations tied to PLA designations, and relevant policy updates affecting laboratory testing and algorithmic assays. Clinical context describes the intended use of the assay (serum biomarker panel with algorithmic risk stratification) and implications for diagnostic workflows. Data not available in the input is noted where applicable.

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Billing Code Overview

CPT code 0003U is a Proprietary Laboratory Analyses (PLA) code that describes the Overa™ (OVA1 Next Generation), also called Multivariate Index Assay, 2nd Generation (MIA2G), produced by Aspira Labs, Inc. and Vermillion, Inc. The test is a biochemical assay of a serum specimen that measures five biomarker proteins and uses algorithmic analysis with patient data and laboratory results to determine a patient’s risk for ovarian cancer.

Service type: Laboratory multivariate index assay using serum biomarker analysis and algorithmic risk calculation
Typical site of service: Clinical laboratory or reference laboratory performing proprietary diagnostic testing

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CPT 0003U: Overa™ (OVA1 Next Generation) Ovarian Cancer Risk Assay

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Billing Code Overview

Service type: Laboratory multivariate index assay using serum biomarker analysis and algorithmic risk calculation
Typical site of service: Clinical laboratory or reference laboratory performing proprietary diagnostic testing

Clinical & Coding Specifications

Clinical Context

A 52-year-old woman presents to her gynecologist with new-onset pelvic discomfort and an adnexal mass identified on pelvic ultrasound. Serum CA-125 is indeterminate. The clinician orders the Overa™ (OVA1 Next Generation; MIA2G) proprietary multivariate index assay to assess the patient’s risk of ovarian malignancy prior to deciding on surgical referral. A phlebotomy draw of a serum specimen is performed in an outpatient lab or hospital outpatient collection area. The specimen is sent to the performing laboratory (Aspira Labs/Vermillion) for biochemical measurement of five protein biomarkers and algorithmic analysis incorporating patient data (e.g., menopausal status) to generate a risk score. Results are returned to the ordering provider to guide the decision for gynecologic oncology referral or conservative management.

Coding Specifications

Modifier	Description	When to Use
`00`	Default professional and technical components intact	Rarely used explicitly; denotes standard claim when no specific modifier applies
`26`	Professional component	Use if only the interpretation/professional component is billed separately by a physician (uncommon for PLA tests)
`TC`	Technical component	Use when the laboratory bills only the technical/testing component (common for lab performing the assay)
`52`	Reduced services	Use if specimen or testing was limited and the full assay was not performed
`53`	Discontinued procedure	Use if testing process was begun but aborted for clinical reasons prior to completion
`59`	Distinct procedural service	Not listed in the raw modifiers; do not include beyond provided list
`62`	Two surgeons	Use when two qualified providers are involved in a related surgical procedure (not for the lab test itself)
`78`	Return to operating room for a related procedure	Use for surgical care scenarios related to an ovarian procedure, not for the lab test claim
`80`	Assistant surgeon	Use if an assistant surgeon is billed for a related operative procedure
`82`	Assistant surgeon (when qualified resident unavailable)	Use when applicable to related surgery billing
`AS`	Physician assistant, nurse practitioner, or clinical nurse specialist services	Use when a PA/NP provides an associated service that is billable separately
`QK`	Anesthesia medically directed by anesthesiologist with CRNAs	Use only for anesthesia claims tied to a related surgical procedure
`QX`	CRNA service with medical direction by physician	Use only for anesthesia claims tied to a related surgical procedure
`QY`	Medical direction of two, three, or four concurrent anesthesia procedures	Use only for anesthesia claims tied to a related surgical procedure

Taxonomy Code	Specialty	Notes
`207Q00000X`	Obstetrics & Gynecology	Ordering clinicians who evaluate adnexal masses and order ovarian cancer risk assays
`208000000X`	Pathology & Laboratory Medicine	Physicians responsible for laboratory oversight and interpretation of complex assays
`362L00000X`	Clinical Laboratory	Clinical laboratory personnel and organizations performing the technical component of the assay
`2087S0125X`	Gynecologic Oncology	Specialists who receive results to decide on surgical management
`363L00000X`	Independent Laboratory	Labs that may perform proprietary PLA testing and bill the technical component

Related Diagnoses

ICD-10 Code	Description	Clinical Relevance
`N83.20`	Unspecified ovarian cyst	Common finding prompting further evaluation with an ovarian cancer risk assay
`N83.1`	Ovarian torsion	Acute adnexal pathology; not an indication for risk assay but may be present in differential diagnosis
`R10.2`	Pelvic and perineal pain	Symptom that may lead to imaging and subsequent ordering of a risk stratification assay
`N80.0`	Endometriosis of uterus	Endometriosis can cause adnexal masses and elevated CA-125; assay can aid in risk stratification
`C56.9`	Malignant neoplasm of ovary, unspecified	Relevant as the condition the assay is designed to help detect or risk-stratify

Related CPT Codes

CPT Code	Description	Relationship to This Procedure
`0003U`	Overa™ (OVA1 Next Generation), Multivariate Index Assay (MIA2G) — proprietary biochemical serum assay with algorithmic analysis	Primary laboratory test that measures five protein biomarkers and computes ovarian cancer risk
`80053`	Comprehensive metabolic panel	Common ancillary serum testing ordered at same visit to evaluate baseline metabolic status prior to surgical decision-making
`81002`	Urinalysis, non-automated, without microscopy	Common preoperative or clinic evaluation test performed at the same encounter
`36415`	Collection of venous blood by venipuncture	Ancillary CPT often reported by the facility or phlebotomy service for blood draw associated with the assay (billing depends on payer policies)
`88305`	Level IV surgical pathology, gross and microscopic examination	May be performed later if surgical excision of an ovarian mass occurs following high-risk result from the assay

CPT 0001U: PreciseType HEA Multiplex Erythrocyte Antigen Assay

CPT-0002U-POLYPDX-URINE-METABOLOMIC-ASSAY-ADENOMATOUS-POLYP-RISK

CPT 0002U: Urine Metabolomic Polyp Risk Assay (PolypDX™)

CPT-0005U-PROSTATE-EXOSOMEDX-INTELLISCORE-GENE-EXPRESSION-URINE-TEST

CPT 0005U: ExosomeDx Prostate (IntelliScore) Urine Gene Expression Risk Test

CPT-0007U-TOXPROTECT-URINE-DRUG-SCREEN-WITH-DNA-AUTHENTICATION

CPT 0007U: ToxProtect Urine Drug Screen with DNA Authentication

CPT-0008U-H-PYLORI-ANTIBIOTIC-RESISTANCE-SEQUENCING-PANEL

CPT 0008U: H. pylori Antibiotic Resistance Panel, Gene Sequence Analysis

CPT-0009U-DEPARRAY-HER2-FISH-TUMOR-CELL-ISOLATION

CPT 0009U: DEPArray HER2 Tumor‑Cell Isolation with FISH Analysis

CPT-0010U-BACTERIAL-TYPING-WHOLE-GENOME-SEQUENCING-MAYO-CLINIC

CPT 0010U: Bacterial Typing by Whole Genome Sequencing, Mayo Clinic

CPT-0011U-CORDANT-CORE-ALGORITHMIC-ORAL-FLUID-DRUG-TEST

CPT 0011U: Cordant CORE™ Oral Fluid Drug Test with Algorithmic Steady‑State Comparison

CPT-0016U-BCR-ABL1-MAJOR-MINOR-FUSION-TRANSCRIPT-TESTING

CPT 0016U: BCR–ABL1 Major and Minor Breakpoint Fusion Transcript Testing

CPT-0017U-JAK2-MUTATION-UNIVERSITY-OF-IOWA

CPT 0017U: JAK2 Mutation Sequencing (Exons 12–14)

Showing 1–10 of 536

References

1.CPT® Codes and Descriptions. AAPC. Retrieved Jul 29, 2026.
2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Jul 29, 2026.
3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Jul 29, 2026.

Published by

Trek Health powers OpenPayer with a leading commercial payer intelligence platform, seamlessly converting fragmented payer data into actionable insights for provider organizations.

Trek Health ingests and normalizes Transparency in Coverage data and payer policy updates to give provider organizations a clear view of how commercial reimbursement behaves across markets, payers, and services. Our platform transforms raw payer disclosures into structured intelligence that supports contract evaluation, payer negotiations, and service line strategy. By combining market benchmarks with ongoing policy visibility, Trek helps teams identify variability, risk, and opportunity in commercial reimbursement. The result is faster insight, stronger negotiating positions, and more informed financial decisions.

Clinical & Coding Specifications

Clinical Context

Coding Specifications

Modifier	Description	When to Use
`00`	Default professional and technical components intact	Rarely used explicitly; denotes standard claim when no specific modifier applies
`26`	Professional component	Use if only the interpretation/professional component is billed separately by a physician (uncommon for PLA tests)
`TC`	Technical component	Use when the laboratory bills only the technical/testing component (common for lab performing the assay)
`52`	Reduced services	Use if specimen or testing was limited and the full assay was not performed
`53`	Discontinued procedure	Use if testing process was begun but aborted for clinical reasons prior to completion
`59`	Distinct procedural service	Not listed in the raw modifiers; do not include beyond provided list
`62`	Two surgeons	Use when two qualified providers are involved in a related surgical procedure (not for the lab test itself)
`78`	Return to operating room for a related procedure	Use for surgical care scenarios related to an ovarian procedure, not for the lab test claim
`80`	Assistant surgeon	Use if an assistant surgeon is billed for a related operative procedure
`82`	Assistant surgeon (when qualified resident unavailable)	Use when applicable to related surgery billing
`AS`	Physician assistant, nurse practitioner, or clinical nurse specialist services	Use when a PA/NP provides an associated service that is billable separately
`QK`	Anesthesia medically directed by anesthesiologist with CRNAs	Use only for anesthesia claims tied to a related surgical procedure
`QX`	CRNA service with medical direction by physician	Use only for anesthesia claims tied to a related surgical procedure
`QY`	Medical direction of two, three, or four concurrent anesthesia procedures	Use only for anesthesia claims tied to a related surgical procedure

Taxonomy Code	Specialty	Notes
`207Q00000X`	Obstetrics & Gynecology	Ordering clinicians who evaluate adnexal masses and order ovarian cancer risk assays
`208000000X`	Pathology & Laboratory Medicine	Physicians responsible for laboratory oversight and interpretation of complex assays
`362L00000X`	Clinical Laboratory	Clinical laboratory personnel and organizations performing the technical component of the assay
`2087S0125X`	Gynecologic Oncology	Specialists who receive results to decide on surgical management
`363L00000X`	Independent Laboratory	Labs that may perform proprietary PLA testing and bill the technical component

Related Diagnoses

ICD-10 Code	Description	Clinical Relevance
`N83.20`	Unspecified ovarian cyst	Common finding prompting further evaluation with an ovarian cancer risk assay
`N83.1`	Ovarian torsion	Acute adnexal pathology; not an indication for risk assay but may be present in differential diagnosis
`R10.2`	Pelvic and perineal pain	Symptom that may lead to imaging and subsequent ordering of a risk stratification assay
`N80.0`	Endometriosis of uterus	Endometriosis can cause adnexal masses and elevated CA-125; assay can aid in risk stratification
`C56.9`	Malignant neoplasm of ovary, unspecified	Relevant as the condition the assay is designed to help detect or risk-stratify

Related CPT Codes

CPT Code	Description	Relationship to This Procedure
`0003U`	Overa™ (OVA1 Next Generation), Multivariate Index Assay (MIA2G) — proprietary biochemical serum assay with algorithmic analysis	Primary laboratory test that measures five protein biomarkers and computes ovarian cancer risk
`80053`	Comprehensive metabolic panel	Common ancillary serum testing ordered at same visit to evaluate baseline metabolic status prior to surgical decision-making
`81002`	Urinalysis, non-automated, without microscopy	Common preoperative or clinic evaluation test performed at the same encounter
`36415`	Collection of venous blood by venipuncture	Ancillary CPT often reported by the facility or phlebotomy service for blood draw associated with the assay (billing depends on payer policies)
`88305`	Level IV surgical pathology, gross and microscopic examination	May be performed later if surgical excision of an ovarian mass occurs following high-risk result from the assay

CPT 0001U: PreciseType HEA Multiplex Erythrocyte Antigen Assay

CPT-0002U-POLYPDX-URINE-METABOLOMIC-ASSAY-ADENOMATOUS-POLYP-RISK

CPT 0002U: Urine Metabolomic Polyp Risk Assay (PolypDX™)

CPT-0005U-PROSTATE-EXOSOMEDX-INTELLISCORE-GENE-EXPRESSION-URINE-TEST

CPT 0005U: ExosomeDx Prostate (IntelliScore) Urine Gene Expression Risk Test

CPT-0007U-TOXPROTECT-URINE-DRUG-SCREEN-WITH-DNA-AUTHENTICATION

CPT 0007U: ToxProtect Urine Drug Screen with DNA Authentication

CPT-0008U-H-PYLORI-ANTIBIOTIC-RESISTANCE-SEQUENCING-PANEL

CPT 0008U: H. pylori Antibiotic Resistance Panel, Gene Sequence Analysis

CPT-0009U-DEPARRAY-HER2-FISH-TUMOR-CELL-ISOLATION

CPT 0009U: DEPArray HER2 Tumor‑Cell Isolation with FISH Analysis

CPT-0010U-BACTERIAL-TYPING-WHOLE-GENOME-SEQUENCING-MAYO-CLINIC

CPT 0010U: Bacterial Typing by Whole Genome Sequencing, Mayo Clinic

CPT-0011U-CORDANT-CORE-ALGORITHMIC-ORAL-FLUID-DRUG-TEST

CPT 0011U: Cordant CORE™ Oral Fluid Drug Test with Algorithmic Steady‑State Comparison

CPT-0016U-BCR-ABL1-MAJOR-MINOR-FUSION-TRANSCRIPT-TESTING

CPT 0016U: BCR–ABL1 Major and Minor Breakpoint Fusion Transcript Testing

CPT-0017U-JAK2-MUTATION-UNIVERSITY-OF-IOWA

CPT 0017U: JAK2 Mutation Sequencing (Exons 12–14)

Showing 1–10 of 536

References

1.CPT® Codes and Descriptions. AAPC. Retrieved Jul 29, 2026.
2.HCPCS Quarterly Update. Centers for Medicare & Medicaid Services (CMS). Retrieved Jul 29, 2026.
3.ICD-10-CM Full Code List. Centers for Medicare & Medicaid Services (CMS). Retrieved Jul 29, 2026.

Published by

Trek Health powers OpenPayer with a leading commercial payer intelligence platform, seamlessly converting fragmented payer data into actionable insights for provider organizations.

OpenPayer

CPT 0003U: Overa™ (OVA1 Next Generation) Ovarian Cancer Risk Assay

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Billing Code Overview

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CPT 0003U: Overa™ (OVA1 Next Generation) Ovarian Cancer Risk Assay

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Billing Code Overview

Clinical & Coding Specifications

Clinical Context

Coding Specifications

Related Diagnoses

Related CPT Codes

Related Codes

References

Clinical & Coding Specifications

Clinical Context

Coding Specifications

Related Diagnoses

Related CPT Codes

Related Codes

References